On-chip three-dimensional tissue histology for microbiopsies
2016
We report a novel approach that generates a high resolution, three-dimensional (3D) fluorescent staining atlas of tissue microbiopsies in a microfluidic device without destroying the tissue. We demonstrate that this method preserves tissue architecture for multiple murine organs by comparing traditional 2D slices to an optically sectioned 3D H&E-mimic. The H&E-mimic slices show a close qualitative match to traditional H&E. The 3D spatial and molecular information obtainable from this method significantly increases the amount of data available for evaluating both tissue morphology and specific biomarkers in a wide range of both research and clinically driven applications and is amenable to automation.
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