In vitro Cell Compatibility of Prepared Collagen–Glycosaminoglycans–Selenium Substituted Hydroxyapatite Nanopowders Composite Scaffolds

Scaffolds for hepatic tissue engineering were successfully prepared by freeze-drying technique including four types namely; collagenchondroitin sulfate (Co-CS), collagen-sodium hyalurnate (Co-SH), collagen-chondroitin sulfate-selenium substituted hydroxyapatite nanopowders (Co-CS-SeHA2) and collagen-sodium hyalurnateselenium substituted hydroxyapatite nanopowders (Co-SHSeHA2) and using gluteraldehyde as cross-linker to increase the scaffolds strength. The formed scaffolds were characterized by SEM and the results showed porous structured with desirable pore sizes ranging from 73.7 to 103 nm, for hepatocytes culture. The cell compatibility was tested using human bone marrow mesynchymal stem cells (BM-MSCs). Results of fluorescent microscopy of the scaffolds showed that the highest number of cells was on Co-CS-SeHA2 scaffold. The cells were distributed in groups which suggested they proliferated on this material. The Co-CS-SeHA2 prepared scaffold can be applied for further liver/ hepatocytes studies.