The role of PFOS on triclosan toxicity to two model freshwater organisms

Surface-active substances may enhance the bioavailability of certain pollutants by modifying the permeability of cell membranes. However, they could also interact in a positive manner by increasing toxicity to aquatic organisms. A comparative effects assessment of waterborne exposure to triclosan (TCS) alone vs. combined with perfluorooctanesulfonic acid (PFOS) was herein investigated in daphnids (Daphnia magna) and medaka (Oryzias latipes) early life stages (ELS) using a battery of non-invasive behavioral, physiological and anatomical endpoints. Additionally, TCS bioaccumulation was evaluated in medaka embryos to help discern differences in effects caused by either, changes in TCS permeability or by a positive interaction with PFOS. The TCS analytical measurements in the medaka ELS exposure media revealed fast dissipation with half-lives < 12 h. The D. magna immobilization and feeding inhibition assays suggested an increased response when TCS (≥200 and 37.50 μg/L, respectively) was co-exposed with PFOS. Concentrations <800 μg TCS/L did not affect medaka ELS. However, exposures to ≥400 μg TCS/L + PFOS had effects on the embryo and eleutheroembryo viability. The morphometric analysis of the embryonic gallbladder area and the oxidative stress, determined in vivo by the manifestation of reactive oxygen species (ROS), revealed a hormetic response in both experimental sets. The TCS + PFOS experimental set generally resulted in increased gallbladder areas and ROS activity levels compared to those quantified in the corresponding TCS set. The bioaccumulation studies in the medaka embryos revealed comparable TCS levels regardless of PFOS presence. Without disregarding any TCS’s enhanced bioavailability caused by the surface-active substance, overall results primarily indicate increased biological effects of TCS due to a potentiation action of PFOS as a binary mixture with TCS.