Protective effect of HMGB1 A box on organ injury in mice with acute pancreatitis

Objective To observe the curative effect of the HMGBl A box(HMGB1 antagon)in the acute pancreatitis mice model and explore the mechanism of H MGB1 A box in protecting organ from injury induced by acute pancreatitis.Methods Acute necrotizing pancreatitis mice model was in-duced by 20% L-Arg intraperitoneal injection.The recombination HMGB1 A box was used in treat-ment after modeling and all biochemical indicator including the AST,ALT,LDH,BUN and Cr were measured.The pathological change of pancreases,lungs,kidneys and livers were observed,and sur-vival rates and time were compared between the control group and treatment group.Results The pan-creas and lung tissue pathologic score of the A box treatment group decreased more significantly 48 h after modeling as compared with SAP group.The kidney and liver pathological changes of the treat-ment group was milder than in the SAP group.The AST value of treatment group was significantly lower than that of SAP group 24 h after modeling.The value of AST,ALT,LDH and Cr of treat-ment group declined more significantly as cornpared with the SAP group 48 h after modeling.The mice survival rate of treatment group(66.7%)was significantly higher than that of SAP group(26.7%).Conclusion The recombination HMGB1 A box iS remarkably protective to pancreatitis and organ inju-ry and it can raise the survival rate of mice with severe acute pancreatitis. Key words: Pancreatitis; High mobility group box protein 1(HMGB1); Mediator of inflammation; Cytokine