Comparison of two methods for detection of HLA-B27 with early and mid-AS patients

Objective: To compare the accuracy,sensitivity and specificity in detection of HLA-B27 between the enzyme-linked immunoassay and flow cytometry,to investigate the value in the diagnosis of ankylosing spondylitis.Methods: Using the enzyme-linked immunoassay and flow cytometry,the same blood samples of suspected early and mid-AS patients were detected,and then calculated the sensitivity,specificity and positive and negative predictive value according to the results of two methods.Results: The sensitivity,specificity,positive and negative predictive value of the enzyme-linked immunoassay and flow cytometry was 0.960,0.972,0.967,0.972;0.980,0.986,0.980,0.986,respectively,and accordance rate of the results of the two methods was 98.0%.Conclusion: In contrast with flow cytometry,sensitivity and specificity of the enzyme-linked immunoassay is relatively high,thus the enzyme-linked immunoassay is a time-saving,convenient and accurate method for detection of HLA-B27.In basic level hospitals,it is a practical and reliable method for AS patients with early diagnosis and differential diagnosis,and has bright prospects for clinical application.