The Effects of Fixation, Processing and Evaluation Criteria on Immunohistochemical Detection of Hormone Receptors in Breast Cancer

A task force of the Japanese Breast Cancer Society has proposed a recommendation for adequate eval-uation of hormone receptors in routine practice, in order to standardize handling of tissues, staining tech-niques and scoring systems. As a part of the study, several examinations were conducted to detect the effect of technical problems, including the influence of fixation time and other fixation and processing conditions, on the immunoreactivity for ERa.