[Detection of epidermal growth factor receptor gene mutation in non-small cell lung cancer by allele-specific oligonucleotide-PCR and bi-loop probe specific primer quantitative PCR].

Objective To compare the detection sensitivity of epidermal growth factor receptor (EGFR) mutations between allele specific oligonucleotide PCR(ASO-PCR) and bi-loop probe and specific primer quantitative PCR (BPSP-qPCR).Methods A total of 96 non-small cell lung cancer specimens were selected from West China Hospital from September 2009 to December 2010.ASO-PCR was developed to detect the presence of classical EGFR mutations.A total 39 available specimens were also tested by BPSP- qPCR.Results EGFR mutation detection rate was 30.2％ (26/96) by ASO-PCR.The mutation rate was higher in female than in male patients[45.5％ (20/44) vs.17.3％ (9/52),P =0.003],non-smokers than smokers[ 44.1％ (26/59) vs.8.1％ (3/37),P ＜ 0.001 ] and adenocarcinomas than other subtypes of lung cancer [ 37.0％ ( 27/73 ) vs.8.7％ (2/23),P =0.01 ].Among mutation negative cases by ASO-PCR,BPSP-qPCR increased the rate of detection of 19-del and L858R mutation by 10.3％ (4/39) inadenocarcinomas and non-smoking subset.Overall,the mutation detection rate of BPSP-qPCR was higher than that of ASO-PCR [66.7％ (26/39) vs.41.0％ (16/39),P=0.02].Conclusion BPSP-qPCR has a better detection sensitivity than that of ASO-PCR. Key words: Carcinoma, non-small-cell lung;  Receptor, epidermal growth factor;  Polymerase chain reaction