Results Concerning Immature Swine Oocytes Vitrification using Different Concentration of Cryoprotectant

The aim of this study was to establish a protocol with a higher number of viable oocytes after the vitrification procedure. The oocytes were collected by the follicular punction method from swine prepuberal ovaries. A total of 1792 immature oocytes were cryopreserved stepwise in previtrification (PV) and vitrification (VM) media, containing ethylene glycol (EG) permeating agent, combined with trehalose a non-permeating agent. The cells were preserved in three experimental ways, with three different concentration of cryoprotectant in the VM respectively 30, 40 and 45% EG.