Aerobic bacteriological profile and its antimicrobial sensitivity pattern from blood culture specimens in a tertiary care hospital.

Background : Blood stream infections (BSIs) are important cause of mortality and morbidity and are among most common health-associated infections.Blood stream infection constitutes one the most serious situations with increased cost of care, morbidity, mortality and thus, timely detection and identification of blood stream pathogen is important.Sothe present study was undertaken to describe aerobic bacteriological profile and its antibiotic sensitivity pattern from blood culture specimen in a tertiary care setting. Method: The study was carried out in the Department of Microbiology, Indian Institute of Medical Sciences, Jalna, Maharashtra for a period of 8 months (July 2015 to February 2016). A total 1920 samples were evaluated from clinically suspected cases of bacteremia. Blood was collected depending upon age group with aseptic precaution and inoculated on brain heart infusion broth (BHIB).Subculture were made on blood agar and Mac-conkey agar plates. Organisms were identified and antibiotic sensitivity test of isolates were performed. Result : During 8 month study period out of 1920 blood culture, 369(19.21%) yielded growth of different organisms. Out of this 369 organisms gram positive bacteria 199 (53.9%) were isolated more often than gram negative bacteria 170 (46.1%). Staphylococcus aureus 49.05 % was leading pathogens isolated followed by enterobactericae group 21.00% ( Escherichia coli and Klebsiella spp) and Salmonella typhi & S. Paratyphi A 13.82%.          Conclusion: This study provides information on antibiotic sensitivity pattern of blood isolates which may be useful to guide clinicians to initiate empiric therapy and will help in formulation of antibiotic therapy strategy in this part of country. DOI: 10.21276/apalm.2017.988