Multiparameter Single Cell Biosensor for Drug Screening

This chapter elaborates the multiparameter single cell biosensor for drug screening. Drug screening is one of the most cost intensive steps in development of new drugs. Mostly, animals are used for the screening process. Whole cell biosensors are a reasonable way for drug screening. In this study, cells of different lines are fixed on a biosensor device. The positioning of different cell lines on the biochip is performed with the photonic tweezers technology using the light pressure of a near infrared laser. It is shown, that up to ten laser beams are able to pick selected cells, and move them to preselected places on a biochip simultaneously. This system allows a very rapid production of cell biosensor devices with excellent reproducibility. The process works automatically. The effect of drugs on several cell lines is examined simultaneously on one biosensorchip using fluorescence spectroscopy, such as the measurement of the intracellular calcium concentration and intracellular pH.