On-Chip Fluorescence Microscopy for Wide Field-of-View High-Throughput Phynotype Screening of Caenorhabditis elegans
2013
We developed a low-cost compact on-chip fluorescence imaging platform, termed the Fluorescence Talbot
Microscopy (FTM), which utilizes the Talbot self-imaging effect to enable efficient fluorescence imaging over a large
and directly-scalable field-of-view (FOV). The FTM prototype has a resolution of 1.2 μm and an FOV of 3.9×3.5mm^2. We demonstrate the use of the FTM prototype in performing high-throughput imaging of live Caenorhabditis elegans (C. elegans), and RNA interference (RNAi) phenotyping of distal tip cell (DTC) migration defects in C. elegans.
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