Efficient and new method for Tectona grandis in vitro regeneration

Abstract: The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seed - lings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA 3 . With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA 3 during regeneration, mg L -1 ) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA 3 during regeneration, mg L -1 ) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species. Key words : Adventitious rooting, plant growth regulator, plant tissue culture, woody plant.