The remarkable role of shikimic acid pathway in humic acid formation during biochar and montmorillonite addition composting

Abstract The final products of shikimic acid pathway, aromatic amino acids (AAA), can be used as humic acid (HA) precursors. Therefore, the aim of this study was to explore the contribution of shikimic acid pathway on the formation of HA during composting. Four composting treatments were carried out in this study, including the control, biochar addition, montmorillonite addition, biochar and montmorillonite combined addition. The results showed that the correlations between AAA and HA were enhanced during combined addition composting, and functional microorganisms involved in the shikimic acid pathway increased. In addition, random forest model suggested that 63.3% of the top 30 genera contributing to the HA formation were functional microorganisms involved in the shikimic acid pathway, which fully proved the critical role of shikimic acid pathway. Therefore, this study provided a new perspective for revealing the crucial factors that promoted the formation of HA during composting.