Гемодинамическая модель инфицированной раны мягких тканей при дозированной экзодермотензии по данным лазерной допплеровской флоуметрии

The purpose of this paper is to create a hemodynamic model of an infected wound at dosed exodermotension in different phases of wound process and to measure the safe level of exertion applied to wound edges. The experimental study of 16 white outbread male rats weighted 273 ±35 g, which were divided into groups by method of randomization. Hair was removed on the upper back of an anesthetized animal and a contour was applied through a stencil by a marker in the form of circumference of 18 mm in diameter. After that a skin wound was made with a scalpel and underlying muscle tissue was smashed with forceps. To simulate exodermotension, traction is performed by thread passed behind a needle placed closer to a short edge of a frame. Herewith peripheral skin areas within the frame boundaries are subjected to distraction. The skin tension force was controlled by dynamometric data at knot fixation moment. All animals were divided into two groups depending on a wound process phase during exodermotension. In the first group such designed device was applied as soon as a wound has been simulated and in the second group it was used on the third day of uncomplicated course of wound process. The tensile area state was controlled by laser Doppler flowmetry (LDF) with using device ЛАКК-02. 24 hours later the start of dermotension after the animal withdrawal from experiment some tissue sections were taken in distracted flap of 1x2 cm. Cuts were painted with hematoxylin, eosin and picrofuxine under Van-Gizon, by fuxelin. As comparing spare capacities of MC systems in the first and second phase of the wound process it was detected that a maximum load limit to distracted flap was statistically significantly lower in the inflammation phase.