Deep sequencing for cholecystokinin a receptor gene to get loci associated with schizophrenia

OBJECTIVE: To find the risk loci on cholecystokinin A receptor gene (CCKAR) - a schizophrenia candidate gene by using the deep sequencing and then analyze the variations. METHODS: In the present study, 8 schizophrenia patients and 8 healthy controls were recruited. After DNA extraction from peripheral blood, we conducted deep sequencing on CCKAR region by HaloPlex Target Enrichment System (Agilent). We used Unphased software to exclude the false positive. RESULTS: After deep sequencing, we got 103 loci, among which 30 were located in CCKAR gene. Besides, the SNP rs191275118 was found to be associated with schizophrenia. CONCLUSIONS: A new variation that may be associated with schizophrenia was found. The deep sequencing is effective to find genetic variation.