Culdoscopic recovery of human follicular ova and maturation in vitro. (Abstract only)

Recent advances in the in vitro culture of mammalian ova have opened opportunities for experimental investigation of human ovum maturation and fertilization. Then a method is needed whereby a plentiful supply of normal human ova can be made available for study. Most workers used a laparoscopic approach for collecting oocytes to the ovaries of women who could not conceive due to pelvic or Fallopian tube pathology. Although productive of relatively large numbers of oocytes laparoscopy is rather technically troublesome than culdoscopy. Thus the purpose of this study is to determine whether ovarian follicular aspiration at culdoscopy would provide sufficient numbers of oocytes to permit adequate in vitro studies including preliminary testing of culture media for in vitro fertilization.(AUTHORS)