Component resolved diagnosis in real life: the risk assessment of food allergy using microarray-based immunoassay.

Summary Background. The development of component-resolved diagnostics constitutes a potential breakthrough in food allergy testing, as detection of specific IgE (sIgE) to individual allergens may make it possible to establish the risk of a mild versus severe reaction. Objective. To compare allergists’ risk assessment based on the current decision making process with that of virtual allergen-oriented risk assessment through microarray-based immunoassay. Patients and Methods. An observational, real-life study was performed on 86 adults with food allergy. The prescription of epinephrine was the surrogate marker of a severe reaction. In the same patients, the prescription of epinephrine based on the current decision making of the allergist and the independently established allergen-oriented risk assessment determined by microarray-based immunoassay were compared. Results. Fair degree of agreement between the specialists’ risk assessment and that of the microarray-based immunoassay (k index 0.372 (95% CI: 0.1850.559) p < 0.001) was documented. Three causes of discrepancy emerged: the poor sensitivity of the allergen microarray-immunoassay (51.9%), the differences in risk assessment established by the specialist and the microarray-immunoassay (33.3%), the non-inclusion of the causative allergen in the microarray-immunoassay platform (14.8%). Conclusion. Improvement of the diagnostic accuracy of microarray-immunoassay, combined with marrying its results to clinical information, could one day soon lead to changes in clinical practice in food allergy.