Ureasil-Based Polymer Matrices As Sensitive Layers for the Construction of Amperometric Biosensors

Ureasil and ureasil-chalcogenide glass composites of different history (fresh and aged during 1 year) were used for the immobilization of laccase and the construction of amperometric biosensors. A correlation between the microscopical free-volume of the polymer matrices as revealed by low-temperature positron annihilation lifetime spectroscopy and biosensor characteristics of the laccase-containing ureasil based biosensors is established. The observed findings could be applied for improvement of the operational parameters of the constructed biosensors, which may have potential for monitoring the level of pollution of wastewater.