Cardioprotective effects of recombinant human extracellular-superoxide dismutase type C in rat isolated heart subjected to ischemia and reperfusion.

The cardioprotective effect of recombinant human extracellular-superoxide dismutase type C (rh-EC-SOD C) was studied in isolated perfused rat heart subjected to left coronary artery ligation for 30 or 60 min followed by 30-min reperfusion. A comparison was made with the effects of bovine CuZn-SOD. Reperfusion after 30-min coronary artery ligation was associated with a release of creatine kinase (CK) into the coronary effluent (71 ± 5.2 IU/30 min), which was markedly reduced (39 ± 5.5 IU/30 min) in hearts perfused with rh-Ec-SOD C (28 mg/L). CuZn-SOD (4 or 20 mg/l) or a lower concentration of rh-Ec-SOD C (5.6 mg/l) did not significantly attenuate CK outflow during reperfusion, however