Abstract 19671: Therapeutic Angiogenesis of Human iPS Cell-Derived Endothelial and Mural Cells Incorporated With Gelatin Sponge Scaffold in a Murine Hindlimb Ischemia Model

Background: To standardize stem cell-based therapeutic angiogenesis for peripheral artery disease, a transplantation strategy to achieve efficient and sustainable therapeutic effect is required. In this study, we have investigated therapeutic effects of human induced pluripotent stem cell (hiPSC)-derived endothelial cells (ECs) and vascular mural cells (MCs) incorporated with gelatin sponge (GS) scaffold in a murine hindlimb ischemia model. Methods: A hindlimb ischemia model was established in male 8-week-old NOD/SCID mice. We induced ECs and MCs from hiPSCs using a 2-dimensional high-density culture, respectively. We prepared GS by the homogenization of the aqueous solution of gelatin. We prepared a mixture of induced ECs and MCs (1.0 x106 ECs+1.0 x106 MCs / mouse) with or without GS. We injected them into the ischemic thigh muscles just after induction of ischemia. We compared control group (medium), vehicle group (GS + medium) and treatment group (hiPSC-derived ECs and MCs) with or without GS. Measurem...