Accuracy of intraocular pressure by Tono-Pen XL over amniotic membrane patching in rabbits

Abstract Purpose To report an accuracy of intraocular pressure (IOP) by Tono-Pen XL over amniotic membrane patching in rabbits. Design Experimental study. Methods Seven male albino rabbits were used in this study. After general anesthesia, IOP was measured in both eyes using the Tono-Pen without amniotic membrane. A single layer amniotic membrane was then immediately placed over the rabbit eye, and the IOP measurement was repeated. Results The average IOP without amniotic membrane was 5.8 ± 0.6 mm Hg (mean ± SD). The average IOP through amniotic membrane was 6.0 ± 0.4 mm Hg (mean ± SD). There was no statistical difference in IOP measured on corneas with or without the amniotic membrane ( P = .34). Conclusions The accuracy of IOP measurement by Tono-Pen XL over a single layer of amniotic membrane patching was demonstrated using rabbit eyes. Further studies in human eyes may verify our findings in actual clinical settings.