Subpopulacje limfocytów B we krwi obwodowej u dzieci zdrowych

Abstract B-cells play a key role in the protection against infection, create immune memory and produce specific antibodies. Determination of specific B-cell phenotype with reference to age is important in the classification of immunodeficiency and in assessing response to vaccinations. The aim of this study was to determine by the flow cytometry the percentage and the absolute values of the B-cell subpopulations – in peripheral blood – in healthy children and to compare the obtained values considering the gender and age. The population of B-cells (CD19+) was 17.2% of the total lymphocyte population in children in the age group 3–10 years, 16.2% in the group of 10–15 years, and 11.1% in the 15–19 years age group and did not differ between treatment subgroups. The evaluated values of B-cell subpopulations with immunomodulatory antigens – B-cell receptor (CD5, CD21, CD81), B lymphocytes with bone marrow borne antigens (CD10, CD38), antigens of lymphocyte-T-dependent response (CD27, CD40, CD21+CD40-), activation antigen (CD23) and subpopulations with other antigens showed no differences between the subgroups. Similar results were obtained for the absolute values except for the subpopulation of CD19+CD5+, which was the highest in younger children. The results can be used as a pattern for comparison with the results in patients with impaired immune system. This paper in a broad way describes the distribution of different subpopulations of B-cells in peripheral blood of healthy children older than 3 year.