Dynamic expression of P58IPK in retina of diabetic rats

：Background The molecularbiological mechanisms of diabetic retinopathy(DR)is unclear up to now.Researches haveproved that endoplasmic reticulum stress(ER Stress)-associated factors are elevated inperipheral blood in patients with diabetic retinopathy.and P58 IPK can inhibit thosefactors.So the relationship between P58 IPK and DR is worth to investigate. Objective Theaim of this study was to detect the dynamic expression of P58 IPK in the retina ofdiabetic rats. Methods The diabetic animal models were established in 18 clean male SDrats by intraperitoneal injection of stilptozotiein(STZ)at a dose of 60 mg/kg.The ratswere sacrificed in 1,3,6 months after injection.The expression change of P58 IPK mRNA inthe rats retina was detected by quantitative real-time RT-PCR.Other 6 matched normal ratswere used as control groups.This experiment followed the Regulations for theAdministration of Affair Concerning experimental Animals by State Science and TechnologyCommission. Results The rats showed more drinking,more food and more urine after STZinjection with the blood glucose level≥ 16.5 mmol/L.The success rate of diabetic models was 100%.The Avalue of P58 IPK mRNA/β-actinin rat retina was 0.800±0.005 and 0.975±0.008 after injection of STZ.and that of controlrats was 0.725±0.006,showing statistically significant difference betweenthem(t=22.589,t=62.784,P＜0.05).In 6 months after injection of STZ,the expression of P58 IPKmRNA in experimental diabetic rat retina was evidently lower than the eontrol rats(0.671±0.004versus 0.725±0.006,t=-17.984,P＜0.05).Conclusion P58 IPK has a close relation to the pathogenesisof DR,and it plays a retarding role for DR. Key words: P58 IPK; Endoplasmic retieulum stress; Diabetic retinopathy