Improved method for preventing cross-contamination in amplification technologies nucleic acid
2009
Method to reduce cross-contamination during the amplification reaction cadenade (PCR) of a nucleic acid, comprising the steps of: a. providing a sample solution containing said nucleic acid; b. amplifying said nucleic acid in the presence of an enzyme having DNA activity glicosilasapara generate DNA with at least one abasic site; c. providing at least one reagent facilitating degradation of abasic DNA's being dichoreactivo a polyamine; d. incubating said sample solution under conditions suitable to cause degradation of dichoADN with at least one abasic site.
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