Association of Two Single-Nucleotide Polymorphisms (rs1805087 and rs1801131) with Coronary Artery Disease in Golestan Population

Objectives: New risk factors, such as plasma homocysteine level, have been rencetly recognized as independent risk factors for coronary artery disease (CAD). Mutations in some genes, affecting plasma homocysteine level, may be associated with CAD. Association studies in many populations have demonstrated a significant association between the development of CAD and 2 polymorphisms, rs1801131 and rs1805087. Methods: In this case-control study (case group, 120 cases; control group, 130 controls), performed in Golestan province, Iran, rs1801131 single-nucleotide polymorphism (SNP) was genotyped in 5, 10-methylene tetrahydrofolate reductase (MTHFR), and rs1805087 SNP was genotyped in 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR). Tetraprimer amplification refractory mutation system-polymerase chain reaction (tetraprimer ARMS-PCR) method was applied to analyze these 2 sites. Results: In the rs1801131 site, genotype frequencies of AA, AC, and CC were 51%, 34%, and 15%, respectively in the case group, while the corresponding frequencies in the controls were 43%, 38%, and 19%, respectively. The results indicated that the difference in A and C allele distribution of rs1801131 was not significant among the controls and patients (OR, 1.34). Genotyping of rs1805087 demonstrated no variety at this position in the population. Conclusions: We conclude that the presence of C allele does not increase the risk of CAD in the population of Golestan province. In addition, MTR rs1805087 SNP is not a suitable marker for population-based studies related to CAD. However, further studies are needed in larger populations to confirm these findings.