Estimation of the turnover number of laccase enzyme

The estimation of the enzyme kinetic parameters is always a challenging problem. This paper covers two methods to estimate the turnover number and illustrates these by the example of laccase enzyme. Both methods exploit the dynamic behaviour of the enzyme reaction, one of them uses the derivative values the other the integral ones. The experimental system is characterized which can be described by the simple Michaelis-Menten enzyme reaction scheme. The results of this research are the estimation of the turnover number, the calculation of the total enzyme (which is active in this reaction) and the calibration coefficients between absorbance and molar concentration for the product. This calibration is called calibration by kinetics.