The effects on dermal wound healing using novel peptide modified by bone morphogenic protein-2

New alternatives to growth factors, such as transforming growth factor beta (TGF-β), epidermal growth factor (EGF), fibroblast growth factor (FGF) and bone morphogenetic proteins (BMP), for wound healing have been devised and investigated to maintain their biological properties, as well as to reduce their complicated adverse effects. In this study, we have focused on OP10 peptide modified from BMPs which regulates a variety of cellular processes, such as proliferation, differentiation, bone/cartilage morphogenesis, apoptosis, and wound healing. The wound healing process involves multiple physiological processes, such as proliferation and migration of dermal fibroblasts and epidermal keratinocytes. These processes play an important role in collagen production and the regulation of elastin levels in dermal tissue regeneration. In order to evaluate the promotion of cell proliferation and migration using OP10, MTT and scratch assays were carried out using normal dermal human fibroblast (NHDF). OP10 promoted proliferation and migration of NHDFs similar to those found with FGF. OP10 was focused on this study and was further investigated for its wound skin regeneration capacity and compared to FGF, by mRNA and protein expression. OP10 was found to increase the protein expression of procollagen and the mRNA level of Type I collagen, to levels similar or even higher than that found with FGF. OP10 inhibits not only matrix metalloproteinase (MMP)-1 expression but also elastase secretion, higher than the effects seen with FGF. Based on these results, we conclude that OP10 plays a role in the regeneration of damaged skin by activating dermal fibroblasts in vitro and may have further potential as wound repair or cosmetic materials for wrinkle improvement.