Buckling of RBC under positive and negative driving pressure in a microchannel

Different cell buckling between positive and negative driving pressure in a microfluidic channel is investigated. Red blood cells under three different osmotic conditions have significant differences between positive and negative driving pressures. Cell insertion length is employed as an index for cell buckling. Greater variations of the insertion length are found with negative driving pressure. This work provides critical information for choosing a suitable type of driving pressure in microfluidic applications.