Research for protective effect of pretreatment by flunarizine hydrochlorid on focal cerebral ischemia reperfusion damage in SD rat with hyperglycemia

Objective Applying focal ischemia-reperfusion model of SD rat on the condition of hyperglycemia, through observing the state of neurologic impairment score, cerebral pathomorphology and infarction volume after focal ischemia-reperfusion damage in SD rat on the condition of hyperglycemia. Method :36 healthy male SD rats(weight from 180 g to 220 g) with normal blood glucemia( ＜6 mmol/l)were randomly divided into 2 groups : hyperglycemia group ( n = 18 ) and flunarizine + hyperglycemia group ( namely flunarizine group n =18 ). Each group was divided into 3 subgroups according to reperfusion 3 h ( n = 6 ), 6 h ( n = 6), 24 h ( n = 6 ) after ischemia for 90 minutes. Compare the differences of neurologic impairment score, cerebral infarction volume and pathomorphology. Results 1. There are great difference of the neurologic impairment score between the flunarizine group and hyperglycemia group(P ＜0. 05) ,The infarction volume can be seen at flunarizine group,and the peak was at reperfusion 24 hours. Compared 6 h with 3 h and 24 h with 6 h in flunarizine group, P ＜0. 01. The infarction volume in the flunarizine group was lower than that in the hyperglycemia group,P ＜0. 01 at reperfusion 3 hours and 6 hours, P ＜ 0. 05 at reperfusion 24 hours. Cerebral tissue pathomorphology:In flunarizine group, the injury of cerebral tissue became serious with time went by, but compared with hyperglycemia group, the number of neuron which became degeneration and necrosis decreased, vacuolization and intertissue edema became relieved. Conclusion On the condition of hyperglycemia, pretreatment of flunarizine could decrease focal ischemia-reperfusion damage, relieve the neurologic impairment symptoms; reduce infarction volume, reduce the neuron degeneration, necrosis and tissue edma. Key words: Flunarizine hydrochlorid ; Hyperglycemia; Ischemia reperfusion