Effect of TGRJ on Blood Press,NO and AngII in Renal Hypertensive Rats

Objective:To study the effect of total glycosides of Ranunculus japoniucus(TGRJ) on blood pressure,nitric oxide(NO)calcium and angiotensinⅡ(AngⅡ) in renal hypertensive rats(RHR).Methods:1) RHR were established by two kidney one clip(2K1C) and drugs were given by intragastric administration for 5 week,the blood pressure were measured at the end of 5 week,detected the concentration of NO in the serum and AngⅡ in the blood plasma,heart and kidney tissue.2) Used a new generations of Ca2+ fluorescent probe(Fluo-3/AM) to mark calcium in vascular smooth muscle cells.Observed the fluorescence imaging by inverted fluorescence microscope and measured the fluorescence intensity of calcium by fluorescence spectrophotometer in vascular smooth muscle cells.Results:1) The high,medium doses of TGRJ could decrease blood pressure of RHR(P0.05),TGRJ could significantly increase the concentration of NO in the serum(P0.01),but it showed no significant effect on the concentration of AngⅡ(P0.05);2) The Ang Ⅱ could significantly promote the calcium ions in extracellular inner flow.The different doses of TGRJ could reduce the calcium ions in cells which were mediated by AngⅡ.Conclusion:TGRJ could decrease blood pressure in RHR.the mechanism might be related to increasing the rats′ NO level and reducing the calcium ions level in cells which are increased by AngⅡ.