Isolation, identification and drug sensitivity test of pathogenic bacteria from Palea steindachneri with red abdominal shell disease

【Objective】The pathogen of red abdominal shell disease of Palea steindachneri and its drug sensitivity were studied to provide references for effective prevention and control. 【Method】Pathogen was isolated from liver, heart and ascites using routine methods. Pathogenicity of the strains was determined by artificial infection. Identification of the strains was performed by API 20 NE analysis and 16 S r RNA gene sequencing. Drug sensitivity test was carried out with K-B method. 【Result】Three pathogenic Aeromonas hydrophila strains named NNSR01, NNSR02 and NNSR03 were isolated from ascites, heart and liver tissues from sick Palea steindachneri. The strains shared the highest similarity(99.6%) with the type strain ATCC 7966 of A. hydrophila and they were all sensitive to sulperazone, rocephin, polymyxin B and cefobid, but resistant to cephalosporin VI, penicillin G and ampicillin. 【Conclusion 】The pathogenic bacteria of red abdominal shell disease of P. steindachneri in a turtle farm of Guangxi is Aeromonas hydrophila, which can be treated by sulperazone, rocephin, polymyxin B and cefobid. Besides, breeding density, feeding management and water quality should be paid attention in winter Palea steindachneri greenhouse. To curb prevalence of phytosis like red abdominal shell disease, epidemic prevention and quarantine as well as sick body separation must be strengthened.