Cloning and Sequence Analysis of the RAPD Markers Related to Sex in Siraidia grosvenorii

The molecular markers related to sex locus in Siraidia grosvenorii were screened by using RAPD technique with 90 random primers.3 male specific fragments were amplified by S60 and S90 random primers.305bp,405bp and 746bp sequences were identified through recycle,clone and sequence analysis.The results showed that the specific sequences were found for the first time in Siraidia grosvenorii,which shared a high homology in base sequences with the sex chromosome in animals.This study provided fundation for the further research such as SCAR marker,which could be used for sex identification of seedling in Siraidia grosvenorii.