Additional file 2: Figure S2. of Overexpression of the double homeodomain protein DUX4c interferes with myofibrillogenesis and induces clustering of myonuclei

DUX4c overexpression induces the formation of ring-like clusters of nuclei and detection of DUX4c in nuclei and the cytoplasm. Healthy primary myoblasts were transfected with pAC1M2-DUX4c and differentiated 48 h later. After formation of the first myotubes (day 3), DUX4c expression was induced by the addition of doxycycline (0 ng, 100 ng, or 1 μg) to the culture medium. At day 8, myotubes were fixed. DUX4c (red) and troponin T (green) were detected by immunofluorescence. Nuclei were labeled with DAPI. The circle indicates the cytoplasmic detection of DUX4c at one myotube tip, where troponin T expression was highest in the elongated myotubes. Scale bar: 30 μm. (PDF 89 kb)