Enteric virus levels in river water

Abstract During a survey conducted from January 1988 to December 1989, enteric virus levels in river water ranged from 13 to 192 plaque forming units per liter (pfu/l) on a monthly average. Enteric virus levels were high in winter (Jan. to Mar.) and summer (Jun. to Aug.). Isolation of polioviruses coincided with the administration of polio vaccine. Coxsackie group B viruses, particularly type 4, were isolated over a long period in 1988 and 1989. Echoviruses and adenoviruses were isolated throughout the year, though in small numbers. Reoviruses were isolated most frequently throughout the year. Among the five cell lines used in virus isolation. HEp-2 cells were the most sensitive to polioviruses, coxsackie group B viruses and adenoviruses. RD-18S and Vero cells were useful for isolation of echoviruses and reoviruses, respectively.