ANTAGONISTIC POTENTIALITY OF SECONDARY METABOLITE PRODUCING ACTINOMYCETES ISOLATED FROM SOIL OF WESTERN RAJASTHAN

In present study soil samples were collected from different areas of Western Rajasthan. 20 isolates of actinomycetes were isolated on Actinomycetes Isolation Agar (AIA). Several biochemical and physiological tests were performed for identification of these isolates. These isolates were screened for antagonistic potentiality against gram‐positive and gram negative human pathogenic bacteria. Isolates were cultivated in fermentation media for 7 days to extract out secondary metabolites of antimicrobial nature. Solvent extraction method was used to purifying antimicrobial compounds from filtrate. Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Citrobacter koseri were used as test pathogen. Only isolate no. 18 was found to be effective against most of the pathogens. Maximum activity was recorded against Staphylococcus aureus and minimum activity against Citrobacter koseri where as no activity was recorded against Escherichia coli. From all the results we concluded that Actinomycetes isolates showed significant antagonism against test pathogens.