Elimination effects of toxic trans-crotonaldehyde in mitochondria of glioma by lithocholic acid

AbstractThe purposes of this study were to explore the factors contributing to the generation of the toxic trans-crotonaldehyde in mitochondria of glioma and to look for the elimination effects of the toxic trans-crotonaldehyde by the lithocholic acid. Ultraviolet-visible absorption spectroscopy and mitochondrial isolation and extraction from glioma were used in this study. Research findings showed that the factors contributing to the generation of the toxic trans-crotonaldehyde were hydrogen peroxide, mitochondria of glioma, and their appropriate interaction, respectively. The appropriate quantity of the toxic trans-crotonaldehyde could be induced by hydrogen peroxide (0.16 mmol), mitochondria of glioma (3 mg protein/ml), and 30 min of their interaction, respectively. The most suitable conditions for the lithocholic acid to eliminate the toxic trans-crotonaldehyde were the weakly alkaline of mitochondria of glioma (pH 8.0), the lithocholic acid (0.15 mmol), and 15 min of interaction, respectively. The co...