Mouse pathway biology models: Investigating the biology ofalternaria alternatainduced lung inflammation in the mouse - Role of ILC2 and Th2 cytokines

In this study, we established a time course for BALf eosinophilia following A. alternata challenge and examined the effects of neutralizing antibodies on Th2 mediated inflammation. We demonstrated that anti-IL-5 and anti-IL-13 antibodies inhibit eosinophilic inflammation (91% ± 2 and 65% ± 6; mean ± SEM, respectively), while anti-IL-4 and anti-TSLP treatment had no significant effect on eosinophil levels. In addition, using flow cytometry, we detected a significant increase in ILC2s presence in the lung, following A. alternata challenge. Finally, our mRNA expression profiling indicate a time dependent induction for a range of immunomodulators including PD-1, PD-L1, PD-L2 and TIGIT, with levels reaching peak at 72 to 96 hours post challenge.