Abstract 129: Assessment of Reperfusion Effects in an Extracorporeal Life Support and Cardiopulmonary Resuscitation Cardiac Arrest Model in Rats by Cerebral Microdialysis

Background: Comparison of ischemia- and reperfusion markers determined by brain microdialysis in a model of two different reperfusion methods after ventricular fibrillation (VF) cardiac arrest in rats. The goal was to establish online measurement of ischemic stress and reperfusion to guide an experimental approach and future therapies. Methods: Male Sprague-Dawley rats were used in a model of 8 minutes VF cardiac arrest. Microdialysis assessment was performed in the right hippocampal CA1 region with 4 baseline and 6 intervention samples. Probe placement was verified histologically after necropsy. Sampling was performed at 1μl/min with 8 minutes intervals. Lactate and pyruvate were assessed. Lactate/pyruvate ratios, reflecting ischemia, were calculated during extracorporeal life support (ECLS), with cardiopulmonary bypass and were compared to cardiopulmonary resuscitation (CPR). A sham group was used for baseline assessment. In this preliminary analysis one animal was used in each group. Results: Return of...