Effects of carotenoids on the absorption and fluorescence spectral properties and fluorescence quenching of Chlorophyll a

Abstract Fluorescence and absorption characteristics of Chlorophyll a (Chl-a) were modulated by the carotenoids (Cars) with different numbers of conjugated carbon‑carbon double bonds in solutions. The Chl-a absorption appears the redshift phenomenon with the effective conjugated of Cars increasing. The absorption of Chl-a and Cars are linearly dependent on intrinsic factors, namely effective conjugate length and functional groups, and on environmental factors, such as the polarizability of the solvent. Cars can be able to quench the Chl-a fluorescence by producing the non-emitting exciplex intermediate. The effective conjugated length of Cars is one of the reasons that effect the fluorescence quenching of Chl-a. According to the Stern-Volmer plots, the Chl-a fluorescence quenching should be predominantly dynamic rather than static.