Genotyping‐by‐Sequencing

Genotyping-by-sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high-throughput sequencing. GBS is a refinement of restriction site–associated DNA sequencing (RADseq) methods, with a goal of being able to perform library preparations quickly, cost-effectively, and in a high-throughput manner. This protocol contains the steps necessary to go from purified DNA to Illumina-ready libraries. It also covers the considerations that go into planning a GBS experiment. © 2017 by John Wiley & Sons, Inc. Keywords: GBS; genotyping; high-throughput sequencing; RADseq; restriction enzyme