Palmitate up-regulates laminin expression via ROS/integrin αvβ3 pathway in HLSECs

// Qi Zhang 1 , 2 , * , Luxia Jiang 3 , * , Jing Yu 4 , Limin Tian 1 , Tiankang Guo 1 , Baoshan Di 5 , Jinxing Quan 1 , Jing Feng 1 and Jing Liu 1 1 Department of Endocrinology, Gansu Provincial Hospital, Lanzhou City 730000, Gansu Province, China 2 School of Basic Medical Sciences, Lanzhou University, Lanzhou City 730000, Gansu Province, China 3 Intensive Care Unit, Lanzhou University Second Hospital, Lanzhou City 730000, Gansu Province, China 4 Gansu University of Chinese Medicine, Lanzhou City 730000, Gansu Province, China 5 Emergency Department, Gansu Provincial Hospital, Lanzhou City 730000, Gansu Province, China * These authors contributed equally to this work Correspondence to: Jing Liu, email: liujing551108@126.com Keywords: human liver sinusoidal endothelial cells; integrin αvβ3; laminin Received: September 14, 2017     Accepted: March 24, 2018     Published: June 25, 2019 ABSTRACT Aims/Introduction To investigate the roles of reactive oxygen species (ROS) and integrin αvβ3 in palmitate-induced laminin expression of human liver sinusoidal endothelial cells (HLSECs). Results The protein expression of integrin αv, integrin β3 and laminin are increased by palmitate in HLSECs in a time- and dose-dependent manner. NAC, the ROS inhibitor, significantly inhibited the up-regulation of protein expression of integrin αv, integrin β3 and laminin by palmitate ( P < 0.05). Palmitate markedly enhanced ROS formation ( P < 0.05), which was not inhibited by LM609, the antibody of integrin αvβ3. Palmitate significantly increased laminin synthesis ( P < 0.05), which was attenuated by LM609 and NAC ( P < 0.05). Materials and Methods HLSECs were treated with palmitate in the presence or absence of LM609 (10 μg/ml) or N-acetylcysteine (NAC) (2 mM). Expression of integrin αv, integrin β3 and laminin were measured by RT-PCR and Western blot. Immunocytochemistry were used for examining laminin expression. The generation of ROS was measured using the fluorescent signal 2',7' dichloro-fluorescein diacetate (DCFH-DA). Conclusions The results suggested that palmitate increases laminin expression through ROS/integrin αv/β3 pathway.