[Effect of sanshui baihu decoction on the proliferation of fibroblast-like synoviocytes and its secretion of IL-6 and IL-17].

Objective To observe the effect of Sanshui Baihu Decoction(SBD) containing serum on the proliferation of in vitro cultured fibroblast-like synoviocytes(FLS)derived from rheumatoid arthritis(RA) and osteoarthritis(OA) and its secretion of interleukin-6(IL-6) and IL-17,and to explore the pharmacological mechanism of SBD.Methods The FLS obtained from cultured RA and OA patients' synovial tissue were cultured and passaged in vitro in a routine way.The cultured medium was changed to DMEM with 20% SBD containing serum and cultured for 72 h after cultured for 3 to 6 generations.The proliferation rate of FLS was detected by MTT assay.Levels of IL-6 and IL-17 in the supernatant were detected by ELISA.Leflunomide and saline containing serum were used as positive and negative control respectively.Results SBD containing serum significantly inhibited the proliferation of RA-FLS and OA-FLS,and decreased the secretion of IL-17 in RA-FLS.Its inhibition efficiency of SBD was equivalent to that of Leflunomide.No obvious inhibition on the secretion of IL-6 in RA-FLS was observed.It had no significant effect on the secretion of IL-17 and IL-6 in OA-FLS.Conclusion SBD could inhibit the proliferation of FLS and the secretion of IL-17 in RA-FLS,which might be one of its pharmacological mechanisms for treating RA.