Abstract 2495: Quantitative screening of protein biomarkers of hepatocellular carcinoma by antibody array

Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Protein biomarkers can be key indicators for evaluating the presence or progression of a human disease or therapeutic response to treatment. We recently carried out a biomarker screening in hepatocellular carcinoma (HCC) using our antibody array platform. Using 25 pairs of HCC and adjacent control tissue samples, the expression levels of 274 proteins including growth factors, inflammation factors, angiogenesis, apoptosis factors and adhesion molecules, were examined using an antibody arrays. A panel of proteins demonstrating significant differences in expression levels between HCC and adjacent control samples was identified by significance analysis of microarrays (SAM) and Wilcoxon signed-rank test, combined with fold-change determination. Additionally, proteomic analysis algorithms were used to capture GO annotations and to construct signaling pathways and protein-protein interaction networks for these proteins. Proteins selected through these comprehensive analyses were designated as potential biomarkers and were well able to distinguish hepatocellular carcinoma tissue samples from paired, adjacent controls with cluster tools. The expression levels of several proteins were further evaluated by enzyme-linked immunosorbent assay (ELISA). Finally, we performed follow-up verification studies on selected proteins (Nidogen-1 and CEACAM-1) for detection of HCC in 164 pairs of serum samples from HCC patients and normal subjects with reverse phase protein array (RPPA). Our work reveals promising protein biomarkers for detection of HCC. Citation Format: Shuhong Luo, Ruochun Huang, Zhongsheng Wang, Yun-Ru Chen, Jinfei Lin, Ruo-Pan Huang. Quantitative screening of protein biomarkers of hepatocellular carcinoma by antibody array. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2495. doi:10.1158/1538-7445.AM2014-2495