Implementation of the aber biomass probe in Pall's iCELLis nano bioreactor provides a robust and reproducible method to assess cell density

Background & Aim In the past five years, Pall's iCELLis bioreactors have emerged as the leading bioreactor technology for clinical manufacturing of viral vectors and vaccines. Pall and InVitria have previously demonstrated the successful implementation of OptiPEAK HEK293T blood-free media formulation in the iCELLis bioreactor to produce viral vectors in adherent HEK293T, including rapid and uniform cell attachment to the iCELLis fixed-bed bioreactor and robust cell expansion kinetics found to be equivalent to those observed in serum-containing media. However, some questions remain regarding the impact of cell aggregation and fixed bed compaction on cell distribution, transfection efficiency, and subsequent cell productivity. Methods, Results & Conclusion In this poster, we look at the cell productivity as a function of aggregation in the cell seed and fixed bed compaction to better understand bioprocessing mechanisms that contribute to low and high cell productivity. We also demonstrate methods and techniques to enhance the cell production.