Cloning and Nucleotide Sequence Analysis of Complementary Deoxyribonucleic Acid for Bovine Preproinsulin
1987
A cDNA library was prepared from poly(A+) RNA isolated from fetal bovine pancreas. Bacterial colonies were screened for sequences homologous to a rat preproinsulin I cDNA probe. Ten positive clones were selected at random and further studied. Northern blot analyses revealed that seven of these clones hybridized to a single RNA species, of approximately 400 nucleotides. Sequence analysis of one of these clones (pbl2885)revealed the entire structural region of bovine preproinsul in mRNA including a 72 nucleotide region encoding a signal peptide enriched in hydrophobic residues. The overall nucleotide homology between bovine and human preproinsulin mRNA was 76% for the preregion, 89% for the A chain, 83% for the B chain, and 68% for the C peptide (including a 15 nucleotide deletion).
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
25
References
7
Citations
NaN
KQI