Inducing Banana Fusarium Wilt Disease Suppression through Soil Microbiome Reshaping by Pineapple-Banana Rotation Combined with Biofertilizer Application

Abstract. Crop rotation and bio-organic fertilizer application have historically been employed as efficient management strategies for soil-borne disease suppression through soil microbiome manipulation. However, details of how this occurs, and to what extent the combination of methods affects soil microbiota reconstruction from diseased soils lacks investigation. In this study, pineapple-banana rotation combined with biofertilizer application was used to suppress banana Fusarium wilt disease, and effects on both bacterial and fungal communities were investigated using the Miseq Illumine sequencing platform. Our results show that pineapple-banana rotation significantly reduces Fusarium wilt disease incidence, and that the application of bio-organic fertilizer causes additional suppression. Bacterial and fungal communities thrive using rotation in combination with bio-organic fertilizer application: taxonomic and phylogenetic α-diversity in both bacteria and fungi increase along with disease suppression. Between the two strategies, bio-organic fertilizer application affects both bacterial and fungal community composition most predominantly, followed by rotation. Large-scale changes in the fungal community composition and special Burkholderia-related network functions contribute to the observed soil borne-disease suppression. Our results indicate that pineapple-banana rotation combined with bio-organic fertilizer application has strong potential for the sustainable management of banana Fusarium wilt disease.