Biomechamical models constructed by functionalized self-assembing peptide hydrogel and human adipose-derived stem cells using a 3D bio-printer

Objective To observe the growth behavior and multi-directional differentiation capacity of human adipose-derived stem cells (Ad-MSCs) in the tissue engineering models which were constructed by 3D bio-printer with the functionalized serf-assembling peptide hydrogel and AMSC. Methods The Ad-MSCs were isolated and cultured, analyzed and identified by flow cytometry. Thereafter, the tissue engineering models were constructed by 3D bio-printer with the bio-ink which consisted of functionalized serf-assembling peptide hydroge and Ad-MSCs. The Ad-MSCs were induced in tissue engineering models to differentiate into osteoblastic cells and adipocytes. Results The self-assembing peptide hydrogel was observed by an atomic force microscope, and the nanofibers structure with 10-30 nm in diameter and 200-200 nm in length was seen and had the characteristics of nanoscale materials. The cells expressed a cell-surface protein profile positive for CD29 (98.51%), CD90 (97.87%) and CD166 (98.32%) and negative for CD31 (1.58%), CD34 (2.42%), CD45(2.95%) and human leukocyte antigen-DR (HLA-DR, 0.53%). The tissue engineering models were successfully constructed by the mixture solution. Ad-MSCs in the models had a good cellular growth behavior. After osteoinductive differentiation, Alizarin red S staining verified the formation of mineralized nodules.After adipogenic differentiation, Oil red O staining verified the formation of lipid droplets. Neither of the control groups colored. Conclusion Ad-MSCs in the tissue engineering models which were constructed by 3D bio-printer with the mixture solution had a good cellular growth behavior, and also had a strong ability of differentiation. Key words: 3D bio-print; Functionalized serf-assembling peptide hydroge; Adipose-derived stem cells; Tissue engineer