A Novel Dual-Response Chemosensor for Bioimaging of Exogenous/Endogenous Hypochlorite and Hydrazine in Living Cells, Pseudomonas aeruginosa and Zebrafish

Abstract Hypochlorite (ClO-) and hydrazine (N2H4) were poisonous to the biological environment. Accurate and sensitive identification of ClO- and N2H4 in biological and environmental systems was essential to reveal their various physiological and pathological functions. Herein, a dual-response chemosensor, HNTBA, was developed for quantitative monitoring and visualization of ClO- and N2H4 in vitro and in vivo via fluorescence turn-on strategy. With the addition of ClO-/N2H4, fluorescence “OFF–ON” response was obtained for the sensor as a result of specific ClO-/N2H4-triggered C = C bond cleavage reaction with converted to the corresponding aldehyde and hydrazone, which responding mechanism was verified by DFT calculation. Additionally, HNTBA features rapid response (