Rice peptide and collagen peptide prevented potassium oxonate-induced hyperuricemia and renal damage

Abstract The study aimed to investigate the effects and mechanism of rice peptide (RP) and collagen peptide (CP) in reducing uric acid (UA) in hyperuricemia mice. RP and CP were prepared by enzymatic hydrolysis method. The two peptides determined by gel chromatography mothed were mainly distributed between 150 and 1000 Da indicating the small peptides composed of 2–7 amino acids were the main components. The functional evaluation was carried out by establishing hyperuricemia mouse model. The results showed that they suppressed the activities of xanthine oxidase (XOD) and adenosine deaminase (ADA), and showed significantly difference in reducing the activity of ADA. RP, CP and RCP could reduce the reabsorption of UA in renal though inhibit the expression of URAT1 and GLUT9 level and promote the expression of OAT1 level both in gene and protein level. Moreover, the three peptides play an important role in inhibiting renal function injury by reduce the content of BUN and CRE in serum. And, the combination of RP and CP has a better effect than the two peptides alone on preventing the occurrence of hyperuricemia and protecting kidney. These findings suggest that RP and CP have potential application prospects in prevention and therapy of hyperuricemia.