Efficient scarless genome editing in human pluripotent stem cells

Scarless genome editing in human pluripotent stem cells (hPSCs) represents a goal for both precise research applications and clinical translation of hPSC-derived therapies. Here we established a versatile and efficient method that combines CRISPR–Cas9-mediated homologous recombination with positive–negative selection of edited clones to generate scarless genetic changes in hPSCs.